Bone Marrow and Stem Cell Transplantation by Sophie Kusy, Christopher H. Contag (auth.), Meral Beksaç

By Sophie Kusy, Christopher H. Contag (auth.), Meral Beksaç (eds.)

The moment variation of Bone Marrow and Stem phone Transplantation expands upon the former version with present, specified equipment on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and practical characterization of stem cells are incorporated. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and tips about troubleshooting and fending off recognized pitfalls.

Authoritative and useful, Bone Marrow and Stem telephone Transplantation, Second Edition serves as a consultant within the software of molecular tools for regimen or investigational reasons.

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Staining with directly conjugated monoclonal antibodies is performed as follows: Red blood cells (1:100 dilution): glycophorin, CD59 Monocytes (undiluted blood): FLAER, CD64, CD14, CD45 Neutrophils (undiluted blood): FLAER, CD24, CD15, CD45 [35] In order to detect abnormal T-cell populations in patients infected with human T-cell lymphotropic virus (HTLV)-1, antibodies to CD25, CD7, CD3, CD2, CD5, CD8, CD4, and CD45 are used. Abnormal populations of eosinophils can be detected by staining for CD49d, CD33, CD294, and CD45.

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