Biosynthesis and Biodegradation of Wood Components by Takayoshi Higuchi
By Takayoshi Higuchi
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Extra resources for Biosynthesis and Biodegradation of Wood Components
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The direct approach, in which fully developed cells are dissected mechanically into single cell wall layers that can then be analyzed separately. The additive approach was applied by Meier and Wilkie (1959) to pine tracheids and by Meier (1961) to spruce tracheids and birch fibers. During the aging of the young tracheids and fibers in the neighborhood of the cambial zone, successive polysaccharide layers are deposited next to one another by the living protoplasts. In the additive approach, tracheids or fibers are isolated and analyzed at various stages of maturation by total hydrolysis with sulfuric acid.