Advances in Clinical Chemistry Volume 4 by Harry ed. Sobotka

By Harry ed. Sobotka

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Heritable abnormalities resulting in a deficiency of an enzyme, 32 JAMES B. SIDBURY, JR. primarily in the liver, which is necessary for the normal metabolism of the sugar. The melliturias of groups one and two are secondary, incidental, and asymptomatic per se. Some of the melliturias in the last two groups are benign, but others are serious, even fatal, if not correctly diagnosed and properly treated. 4. METABOLISM OF SUGARS The simple sugars glucose, galactose, fructose, and pentoses are not acted on by the digestive enzymes.

SIDBURY, JR. paper. Better separation can be achieved, however, if the solvent is allowed to run off the end of the paper, and the distance traveled by the standards is used for reference. It is convenient to let the chromatograms run overnight and remove and dry them under the hood in the morning. After drying, the chromatogram is sprayed with one of several mixtures which will react with the sugars and develop a color. 1 N oxalic acid) are similar in the color produced with different sugars and in stability of the reagent.

This was especially true when 29 30 JAM= B. SIDBWRY, JR. two or more sugars were present in the urine in varying amounts. With the application of paper chromatography to the separation of sugars, this technique was soon applied to urine, and has developed into the most useful method available ( P1) . Several factors have impeded the progress of detection and study of patients with the rarer forms of mellituria besides the notorious difficulty and unreliability of the methods applicable to the identification of the sugars themselves.

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